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Fundamental Research
Basic research produces knowledge and understanding of molecular mechanisms.
Pharmaceutical Research
We are pharma's dependable and strategic R&D solutions provider supporting early drug discovery programs.
AgroScience and Plant Research
Whether you work on basic plant biology or crop sciences we support you in fostering innovation in your scientific research.
Our services
Yeast 2-Hybrid based Protein Interactions screening
Our ULTImate Y2H™ is the most comprehensive yeast two-hybrid system identifying protein protein interactions as well as DNA, RNA and small bioactive molecule interactions.
Identification of Drug-Target Interactions
Benefit from our unique YChemH screening platform to identify the protein targets of your small bioactive molecule and understand its mode of action
Molecular Glues and Targeted Protein Degradation
Identify protein-protein interactions induced by small molecules with our versatile Y2HChem screening platform, a Y2H-based technology.
Hybribody
Benefit from our proprietary synthetic and humanized VHH library for customized Nanobody® selection/validation and affinity maturation projects.
Resources
Publications
Explore our latest customer publications for insights, inspiration and how-to techniques.
News
Stay tuned for conference news, our newest customer publications and interesting facts and information around our service platforms.
Glossary and Testimonials
Find scientific insights about the Y2H principle, our cutting-edge technologies for VHH selection, validation and affinity maturation as well as testimonials from our customers.
Our libraries
We offer a large variety of high-quality, highly complex yeast two-hybrid libraries available for screening depending on your project aim. Our 25 years’ experience led us to establish a gold standard for library construction and validation, making us a recognized market leader in the field.
Explore our vaste library collection to identify the best fit for your Y2H-based screening or request a custom-made library, starting from your RNA material of choice.
Homo sapiens
Homo sapiens
Cell lines
- B cell lymphoma (mix of SUDHL-4, SUDHL-6, RCK8) ref: [BCLY]
- Bone marrow endothelial cells (HBMEC) ref: [HBMEC]
- Breast tumor epithelial cells (mix of T47D, MDA-MB-468, MCF7, BT20) ref: [HBEC]
- Brown adipocytes (differentiated PAZ6) ref: [PZD]
- Colon tumor epithelial cells (mix of Caco2, HCA7, Colo205, SW480) ref: [HCCA]
- Colorectal carcinoma cells (HCT116) ref: [HCT116TO]
- Ewing's sarcoma cells ref: [HSEG]
- HeLa cells ref: [HeLa]
- Lung tumor cells (mix of A549, H1703, H460) ref: [HLUC]
- Podocytes ref: [HPO]
- Pre-adipocytes (undifferentiated PAZ6) ref: [PZU]
- Prostate tumor cells (mix of PC3, DU145, PNT1A, LNCaP) ref: [HPRC]
- T-cells (CEMC7) ref: [CEMC7]
- Umbilical vein endothelial Cells ref: [HUVEC]
- Umbilical vein endothelial Cells, stressed & unstressed (HUVEC) ref: [HSCUV]
Primary Cells
- Fibroblasts ref: [HF]
- Hepatocytes ref: [PHH]
- Leukocytes + activated mononuclear cells ref: [HLMA]
- Macrophages (mix of Pam3CSK4 induced and IFNß induced) ref: [HMIN]
- Macrophages (non induced) ref: [MAC]
- Melanocytes ref: [HML]
- Thymocytes (mix of CD4+ and CD8+) ref: [HTH]
- Bone marrow-derived hematopoietic stem cells ref: [BMHSC]
Tissues
- Brain adult ref: [HBR]
- Brain fetal (entorhinal cortex and hippocampus) ref: [HFHIT]
- Brain fetal ref: [HFBR]
- Breast ref: [HBT]
- Colon ref: [HCN]
- Heart adult/fetal ref: [HVEH]
- Langerhans islets ref: [HISL]
- Liver ref: [HLV]
- Lung ref: [HNL]
- Muscle skeletal adult/fetal ref: [HMAF]
- Pancreas ref: [HPS]
- Placenta ref: [PLA]
- Reconstituted skin (Episkin) ref: [EpiSkin]
- Retina ref: [HRET]
- Testis ref: [HTestis]
Plants
Arabidopsis thaliana
Tissues
- Flower ref: [ATFO]
- Meiotic bud ref: [ATMB]
- Mixed leaves, TYMV infected, flg22 infiltrated ref: [AIFT]
- Organ boundary cells of inflorescence ref: [ATOBC]
- Rosette leaf, P. syringae infected ref: [AIPS]
- Seedling (one-week old) ref: [ATH]
Barley (Hordeum vulgare)
Tissues
- 7-day old seedling leaf, Blumeria graminis f. sp. Hordei infected ref: [HVMI]
- Seedling leaves ref: [HVSLT]
Brachypodium distachyon
Tissues
- Root and shoot ref: [BMDN]
Corn (Zea mays)
Tissues
- Endosperm ref: [ZMEN]
- Immature ear and tassel inflorescence primordia, vegetative apices ref: [MAET]
- Kernel ref: [ZEMAK]
- Leaf and ovary ref: [MALO]
Grape (Vitis riparia)
Tissues
- Leaf ref: [VIRIF]
Kalanchoe laxiflora
Tissues
- Leaf ref: [KALAX]
Marchantia polymorpha
Tissues
- Mixed tissues ref: [MAPA]
Medicago truncatula
Tissues
- Root ref: [METRO]
- Root, A. euteiches infected ref: [MOTA]
- Whole plant and root, A. euteiches infected ref: [MTA17]
- Medicago truncatula roots mycorrhized ref: [MTRMY]
Melon (Cucumis melo)
Tissues
- Meristem ref: [CUMM]
Morning glory (Ipomoea nil)
Tissues
- Petal ref: [IPON]
Nicotiana benthamiana
Tissues
- Mixed tissues ref: [NIB]
Orange (Citrus sinensis)
Tissues
- Plant, Las infected ref: [CSIL]
Pea (Pisum sativum)
Tissues
- Leaf and stem, ref. [PSLS]
Physcomitrella patens
Tissues
- Protonema and gametophores ref: [PHPA]
Poplar (Populus tremula )
Tissues
- L. x Populus tremuloides (Leaves and Buds) ref: [ASLB]
Rapeseed (Brassica napus)
Tissues
- Leaf, Leptosphaeria maculans infected ref: [BNLI]
Rice (Oryza sativa)
Tissues
- Leaf and root ref: [RLR]
Setaria viridis
Tissues
- Whole plant ref: [SVIN]
Soybean (Glycine max)
Tissues
- Leaf and root, water stressed ref: [GMLR]
- Leaf, shoot, root, B. japonicum inoculated ref: [SSRL]
Tobacco (Nicotiana tabacum)
Tissues
- Leaf ref: [NITA]
Tomato (Solanum lycopersicum)
Tissues
- Fruit ref: [TOFT]
- Leaf, petiole, stem and root, TYLCV infected ref: [TOPLI]
- Leaf, stem and petiole ref: [TOLE]
- Meristem, leaf primordia and stem, floral bud ref: [TOM]
- Ovary ref: [TOV]
- Root, R. solanacaerarum and M. incognita infected ref: [TORI]
Wheat (Triticum aestivum)
Tissues
- Head, leaf and root ref: [WHLR]
- Leaf, Zymoseptoria tritici inoculated ref: [WLIZT]
- Leaves, infected with powdery mildew and wheat leaf rust ref: [TALSI]
- Triticum turgidum (Leaf, peduncle and grain) ref: [TTLPG]
- Wheat infected with Fusarium graminearum ref: [WIFG]
- Triticum aestivum Cadenza, non-infected and infected with wheat stem rust ref: [TAC]
Vitis rotundifolia
Tissues
- Vitis rotundifolia Fruit ref: [MURAF]
Rodents
Mouse (Mus musculus)
Tissues
- Brain adult ref: [AMB]
- Brain embryo (mix of E10.5 and E12.5) ref: [MBE]
- Brain embryo (mix of E14, E16 and E18) ref: [MB6E]
- Mus musculus whole cochlea ref: [MCOC]
- Embryonic stem cells (treated with CHIR and bFGF) ref: [MSCT]
- Embryonic stem cells ref: [MESC]
- Inner Ear E16.5 ref: [MOINE]
- Inner Ear ref: [MIE]
- Kidney adult ref: [MKI]
- Kidney embryo ref: [MOKE]
- Neurosphere adult ref: [MANE]
- Ovary ref: [MMO]
- Pancreatic beta-cells ref: [MPC]
- Spleen ref: [MSPL]
- Testis ref: [MUST]
- Corti Organ ref: [MMCO]
- Mouse Postnatal day 7 lungs ref: [MMPLG]
- Mus musculus whole cochlea ref: [MCOC]
Rat (Rattus norvegicus)
Tissues
- Hippocampus ref: [RHC]
- Rear brain ref: [RBP]
Insects
Drosophila melanogaster
Tissues
- Adult head ref: [DMT]
- Embryo (0-12h and 12-24h) ref: [DME]
- Larvae brain ref: [DMLB]
- Ovary ref: [DMOV]
- Testis ref: [DMTES]
- Third instar larvae ref: [DLTS]
Tiger mosquito (Aedes albopictus)
Tissues
- Larvae cells ref: [AALC]
Green peach aphid
Cells
- Myzus persicae ref: [MYPE]
Nasonia vitripennis
Tissues
- Ovary and Testis ref: [NVOT]
Other Model Organisms
Caenorhabditis elegans
Tissues
- Embryo ref: [CEE]
- Mixed stages ref: [CEMS]
Dictyostelium discoideum
Tissues
- Vegetative, aggregative & slug stages ref: [DD]
Gallus gallus
Tissues
- Lung ref: [GALU]
Xenopus laevis
Tissues
- Embryo ref: [XLE]
Fungi
Cells
- Magnaporthe oryzae ref: [MAOR]
- Botrytis cinerea strain BO5.10 ref: [BOCI]
Parasites
Toxoplasma gondii
Cells
- Strain wild-type RH ref: [TGRH]
Trypanosoma brucei (genomic library)
Cells
- Strain TREU927 ref: [TB]
Wheat Curl Mite
Cells
- Aceria tosichella ref: [WCM]
Schistosoma mansoni
Cells
- Whole Organism ref: [SMAN]
Fish
Medaka (Oryzias latipes)
Tissues
- Embryo ref: [OLE]
Rainbow trout (Oncorhynchus mykiss)
Tissues
- Embryonic male gonads ref: [ONMY]
Zebrafish (Danio rerio)
Tissues
- Embryo ref: [ZFE]
Green Algae
Chlamydomonas reinhardtii
Cells
- Strain CC-124 ref: [CHRE]
Mesotaenium endlicherianum
Tissues
- Meso endlicherianum vegetative cells ref: [MEVE]
Yeast
Schizosaccharomyces pombe (cDNA library)
Cells
- S. pombe ref: [SAPO]
Xanthophyllomyces dendrorhous
Cells
- Cells ref: [XADE]
Bacteria (genomic library)
Caulobacter crescentus
Cells
- Strain NA 1000 ref: [CACR]
Enterococcus faecalis
Cells
- Strain V583 ref: [ENFA]
Escherichia coli
Cells
- Strain K-12 MG1655 ref: [EC]
Helicobacter pylori
Cells
- Strain 26695 ref: [HP]
Mycobacterium tuberculosis
Cells
- Strain H37Rv ref: [MYT]
Pseudomonas aeruginosa
Cells
- Strain PAO1 ref: [PSAE]
Staphylococcus aureus
Cells
- Strain COL ref: [Sa]
Streptococcus pneumoniae
Cells
- Strain G1 type 4 ref: [Sp]
Streptococcus pyogenes
Cells
- Strain M5 Manfredo ref: [SCPY]
- Strain SF370 ref: [SP370]
Clostridium difficile
Cells
- Clostridioides difficile CD196 ref: [CD196]
Acinetobacter baumanii
Cells
- Strain ATCC_17978 ref: [ACIBA]
Yeast
Candida albicans (genomic library)
Cells
- Strain SC5314 ref: [CANA]
Saccharomyces cerevisiae (genomic library)
Cells
- Strain YM955 ref: [SaC]
Homo sapiens
Homo sapiens
Tissues
- Brain Adult MBmate NubG-x
- Brain Embryo MBmate NubG-x
- Colon Adult MBmate NubG-x
- Kidney Adult MBmate NubG-x
- Liver Adult MBmate NubG-x
- Lung Adult MBmate NubG-x
- Small intestine Adult MBmate NubG-x
Cell lines
- HeLa MBmate NubG-x
- Hep-G2 (hepatocellular carcinoma) MBmate NubG-x
- Jurkat T-cells MBmate NubG-x
- LNCaP (prostate adenocarcinoma cells) MBmate NubG-x
- Mammary epithelial cells MBmate NubG-x
Other Model Organisms
Caenorhabditis elegans
Tissues
- Adult MBmate NubG-x
- Eggs MBmate NubG-x
Zebrafish (Danio rerio)
Tissues
- Brain MBmate NubG-x
Dictyostelium discoideum
Tissues
- Whole cell MBmate NubG-x
Drosophila melanogaster
Tissues
- Adult MBmate NubG-x
- Embryo MBmate NubG-x
Rodents
Mouse (Mus musculus)
Tissues
- Brain Adult MBmate NubG-x
- Embryo (11 days) MBmate NubG-x
- Heart Adult MBmate NubG-x
- Kidney Adult MBmate NubG-x
- Testis Adult MBmate NubG-x
Rat (Rattus norvegicus)
Tissues
- Neonatal Cardiomyocytes MBmate NubG-x
Plants
Arabidopsis thaliana
Tissues
- Seedlings (6 days, dark grown & light exposed) MBmate NubG-x
Medicago truncatula
Tissues
- Nodules MBmate NubG-x
Rice (Oryza sativa)
Tissues
- Roots and Nodes MBmate NubG-x
Yeast
Saccharomyces cerevisiae (genomic library)
Cells
- Whole cell MBmate NubG-x
The emerging role of ubiquitylation as a key process for the control of numerous signaling pathways has sparked considerable interest in drug research. The large number of human genes believed to encode ubiquitin conjugating enzymes (UBCs ) and E3 ligases (∼600) allows for the ubiquitylation of specific proteins or families of proteins to be independently controlled.
Targeted protein degradation levers these E3 ligases to selectively degrade a chosen protein of interest via the Ubiquitin Proteasome System. Hereby, a ligand that targets the protein of interest and binds to an identified E3 ligases, with or without small molecules, molecular glues or bifunctional molecules and leads the protein into degradation.
Our established human, full length cDNA E3 ligase library offers huge potential to identify E3 ligases that’s binding your preferred target with or without molecular glues molecule. This new type of library offers the opportunity to establish a new screening process in your Targeted Protein Degradation program and we are here to accompany you on the way. To screen your Protein of Interest (POI) against our human E3 ligase library, we offer two formats: traditional Y2H screens and 1-by-1 assays in a 96-well format, both designed for focused interaction analysis.
Cyclic peptides libraries have shown significant potential when used to screen challenging targets in protein-protein interactions assays. One method for the intracellular generation of cyclic libraries is the split-intein circular ligation of peptides and proteins (SICLOPPS).
We offer a huge collection of cyclic peptides with 14 million clones ready to be screen at genetic saturation with our ULTImate Y2H technology. This offer is a powerful tool for drug discovery, especially for early stage hit discovery against intractable targets. Feel free to request more information and contact our expert to start your project.
