The Protein Interactions Expert



ULTImate Libraries

We are proud to have the largest collection of libraries ready to be screened.

With over 100 libraries from more than 35 species, Hybrigenics Services is proud to have the largest collection available for screening. From human to plants, from bacteria to drosophila, the diversity of our highly complex libraries allows us to serve you whatever your research area is.

We keep constructing new libraries to meet the needs of the research community. We value your feedback, please let us know what you want in our contact form or on Twitter with the hashtag #HGXlibraries.

Our libraries are also the most complex ones on the market and ULTImate Y2H™ cell-to-cell mating is the only technology to screen them exhaustively with an average 10-fold coverage of their complexity.

These protein fragment libraries can be used for all ULTImate screens to discover the partners of a protein, a DNA sequence, a short RNA or a small molecule of interest.

our scientist working

Each ULTImate library we construct is validated by stringent quality controls:

  • We verify a complexity of at least 10 million independent, primary fragments in yeast for our random-primed cDNA libraries. The complexity of the genomic fragment libraries constructed for organisms that contain no or few introns is defined by the size of the genome
  • We sequence hundreds of clones to check the fragment size distribution and ensure that ribosomal RNA, mitochondrial DNA and empty vectors represent less than 10% of all sequences.

Please contact us for more details on a specific library.

  • Homo sapiens
    • Cell lines
      • B cell lymphoma (mix of SUDHL-4, SUDHL-6, RCK8) ref: [BCLY]
      • Bone marrow endothelial cells (HBMEC) ref: [HBMEC]
      • Breast tumor epithelial cells (mix of T47D, MDA-MB-468, MCF7, BT20) ref: [HBEC]
      • Brown adipocytes (differentiated PAZ6) ref: [PZD]
      • Colon tumor epithelial cells (mix of Caco2, HCA7, Colo205, SW480) ref: [HCCA]
      • Colorectal carcinoma cells (HCT116) ref: [HCT116TO]
      • Ewing's sarcoma cells ref: [HSEG]
      • HeLa cells ref: [HeLa]
      • Lung tumor cells (mix of A549, H1703, H460) ref: [HLUC]
      • Podocytes ref: [HPO]
      • Pre-adipocytes (undifferentiated PAZ6) ref: [PZU]
      • Prostate tumor cells (mix of PC3, DU145, PNT1A, LNCaP) ref: [HPRC]
      • T-cells (CEMC7) ref: [CEMC7]
      • Umbilical vein endothelial Cells (HUVEC) ref: [HUVEC]
      • Umbilical vein endothelial Cells, stressed & unstressed (HUVEC) ref: [HSCUV]
    • Primary Cells
      • Fibroblasts ref: [HF]
      • Leukocytes + activated mononuclear cells ref: [HLMA]
      • Macrophages (mix of Pam3CSK4 induced and IFNß induced) ref: [HMIN]
      • Macrophages (non induced) ref: [MAC]
      • Melanocytes ref: [HML]
      • Thymocytes (mix of CD4+ and CD8+) ref: [HTH]
    • Tissues
      • Brain adult ref: [HBR]
      • Brain fetal ref: [HFBR]
      • Breast ref: [HBT]
      • Colon ref: [HCN]
      • Heart adult/fetal ref: [HVEH]
      • Langerhans islets ref: [HISL]
      • Liver ref: [HLV]
      • Lung ref: [HNL]
      • Muscle skeletal adult/fetal ref: [HMAF]
      • Pancreas ref: [HPS]
      • Placenta ref: [PLA]
      • Reconstituted skin (Episkin) ref: [EpiSkin]
      • Retina ref: [HRET]
      • Testis ref: [HTestis]
  • Plants
    • Arabidopsis thaliana
      • Flower ref: [ATFO]
      • Meiotic bud ref: [ATMB]
      • Mixed leaves, TYMV infected, flg22 infiltrated ref: [AIFT]
      • Organ boundary cells of inflorescence ref: [ATOBC]
      • Rosette leaf, P. syringae infected ref: [AIPS]
      • Seedling (one-week old) ref: [ATH]
    • Barley (Hordeum vulgare)
      • 7-day old seedling leaf, Blumeria graminis f. sp. Hordei infected ref: [HOIM]
    • Corn (Zea mays)
      • Endosperm ref: [ZMEN]
      • Immature ear and tassel inflorescence primordia, vegetative apices ref: [MAET]
      • Leaf and ovary ref: [MALO]
    • Kalanchoe laxiflora
      • Leaf ref: [KALAX]
    • Medicago truncatula
      • Root, A. euteiches infected ref: [MOTA]
      • Whole plant and root, A. euteiches infected ref: [MTA17]
    • Morning glory (Ipomoea nil)
      • Petal ref: [IPON]
    • Nicotiana benthamiana
      • Mixed tissues ref: [NIB]
    • Orange (Citrus sinensis)
      • Plant, Las infected ref: [CSIL]
    • Physcomitrella patens
      • Protonema and gametophores ref: [PHPA]
    • Rice (Oryza sativa)
      • Leaf and root ref: [RLR]
    • Soybean (Glycine max)
      • Leaf and root, water stressed ref: [GMLR]
      • Leaf, shoot, root, B. japonicum infected ref: [SSRL]
    • Tobacco (Nicotiana tabacum)
      • Leaf ref: [NITA]
    • Tomato (Solanum lycopersicum)
      • Fruit ref: [TOFT]
      • Leaf, petiole, stem and root, TYLCV infected ref: [TOPLI]
      • Leaf, stem and petiole ref: [TOLE]
      • Meristem, leaf primordia and stem, floral bud ref: [TOM]
      • Root, R. solanacaerarum and M. incognita infected ref: [TORI]
    • Wheat (Triticum aestivum)
      • Head, leaf and root ref: [WHLR]
      • Leaf, Zymoseptoria tritici inoculated ref: [WLIZT]
      • Leaf, peduncle and grain ref: [TALPG]
  • Rodents
    • Mouse (Mus musculus)
      • Brain adult ref: [AMB]
      • Brain embryo (mix of E10.5 and E12.5) ref: [MBE]
      • Brain embryo (mix of E14, E16 and E18) ref: [MB6E]
      • Embryonic stem cells (treated with CHIR and bFGF) ref: [MSCT]
      • Embryonic stem cells ref: [MESC]
      • Inner Ear ref: [MIE]
      • Kidney adult ref: [MKI]
      • Kidney embryo ref: [MOKE]
      • Neurosphere adult ref: [MANE]
      • Ovary ref: [MMO]
      • Pancreatic beta-cells ref: [MPC]
      • Spleen ref: [MSPL]
      • Testis ref: [MUST]
    • Rat (Rattus norvegicus)
      • Hippocampus ref: [RHC]
      • Rear brain ref: [RBP]
  • Insects
    • Drosophila melanogaster
      • Adult head ref: [DMT]
      • Embryo (0-12h and 12-24h) ref: [DME]
      • Larvae brain ref: [DMLB]
      • Ovary ref: [DMOV]
      • Testis ref: [DMTES]
      • Third instar larvae ref: [DLTS]
    • Tiger mosquito (Aedes albopictus)
      • Larvae cells ref: [AALC]
  • Other model organisms
    • Caenorhabditis elegans
      • Embryo ref: [CEE]
      • Mixed stages ref: [CEMS]
    • Dictyostelium discoideum
      • Vegetative, aggregative & slug stages ref: [DD]
    • Xenopus laevis
      • Embryo ref: [XLE]
  • Parasites
    • Toxoplasma gondii
      • Strain wild-type RH ref: [TGRH]
    • Trypanosoma brucei (genomic library)
      • Strain TREU297 ref: [TB]
  • Bacteria (Genomic)
    • Enterococcus faecalis
      • Strain V583 ref: [ENFA]
    • Escherichia coli
      • Strain K-12 MG1655 ref: [EC]
    • Helicobacter pylori
      • Strain 26695 ref: [HP]
    • Mycobacterium tuberculosis
      • Strain H37Rv ref: [MYT]
    • Pseudomonas aeruginosa
      • Strain PAO1 ref: [PSAE]
    • Staphylococcus aureus
      • Strain COL ref: [Sa]
    • Streptococcus pneumoniae
      • Strain G1 type 4 ref: [Sp]
    • Streptococcus pyogenes
      • Strain M5 Manfredo ref: [SCPY]
      • Strain SF370 ref: [SP370]
  • Fishes
    • Medaka (Oryzias latipes)
      • Embryo ref: [OLE]
    • Rainbow trout (Oncorhynchus mykiss)
      • Embryonic male gonads ref: [ONMY]
    • Zebrafish (Danio rerio)
      • Embryo ref: [ZFE]
  • Yeasts
    • Candida albicans (genomic library)
      • Strain SC5314 ref: [CANA]
    • Saccharomyces cerevisiae (genomic library)
      • Strain YM955 ref: [SaC]
    • Schizosaccharomyces pombe (cDNA library) 
      • ref: [SAPO]
  • Green algae
    • Chlamydomonas reinhardtii
      • Strain CC-124 ref: [CHRE]
  • Structurally constrained peptides
    • Cysteine-rich ref: [C5_PEP]
    • Proline-rich ref: [C8_PEP]
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