For most of the services we offer we would need to get a DNA template: ULTImate Y2H , ULTImate Y2H+1 , ULTImate Y1H , Loss of Affinity Mutant Screen , 1-by-1 Y2H & Interaction Domain Mapping , 1-by-1 with HTRF® , 1-by-1 with Biacore® .
We recommend sending 5µg of purified DNA of kit purified DNA at 200-400ng/µl in water or Tris (with no EDTA) or as a dry pellet (not on a filter paper).
For ULTImate YChemH projects, we handle the synthesis of your derivatized molecule. You don’t need to provide compounds.
For ULTImate screening projects, we use our own libraries as they are optimized for our process so you don’t need to send a cDNA library. Just choose one from our list. However, we can prepare a custom cDNA library from RNA samples you provide.
In that specific case, we generally ask to receive 1 mg of total RNA, as we expect a yield of 1% to 5% polyA+ RNA after extraction. With 10 to 50 µg of polyA+ RNA, we can attempt several shots of cDNA synthesis if needed.
For specific projects with in vitro interaction experiments, HTS compound library screening or hit to lead services, you may need to send protein samples or compounds in well plates. Contact us for more details .
If you are sending us DNA samples, you can simply send them in tubes with Parafilm by normal ground mail. They don’t need to arrive quickly as DNA is stable.
If you are sending us RNA or protein samples, please use a large package filled with a lot of dry ice and ship it by express mail. This type of material is very fragile and must be kept at least at -20°C. We recommend that you don’t send your parcel before a week-end to make sure that we will be present to immediately store the samples at the appropriate temperature.
If you are sending a small number of compounds; you can ship powders at room temperature by normal ground mail. If we need to receive many compounds, we will require compounds in DMSO in 96 well plates on dry ice (Micronic or equivalent).
Please address your template to our labs at the following address:
3-5 Impasse Reille 75014 Paris