- Interaction Discovery
- Target Deconvolution
- Our libraries
- Our references
- Resource center
ULTImate YChemH is a direct drug target deconvolution method based on the established yeast two-hybrid (Y2H) technique. The small molecule of interest or drug is used as a bait to screen highly complex protein domain libraries prepared from any cell type, tissue or organism. The Drug- Protein interactions are detected thanks to the reconstitution of an active transcription factor from DNA Binding Domain (DBD) and Activation Domain (AD) moieties.
Three components are used:
When a "Drug–Target protein" interaction takes place, the Tagged Drug bridges the gap between the DBD and the AD thanks to the Tag-ABD interaction. This enables the expression of the reporter gene and subsequent yeast growth on a selective medium. Positive clones are then analyzed by sequencing to identify the protein partners.
More details on this technology can be found on our resource center.
The results of your ULTImate YChemH screen come fully analyzed to help you focus on the most relevant interactions. The deliverables include the list of identified protein partners, the experimental interaction domain on each protein and a confidence score.
For any question on this drug discovery service, contact us.