The Protein Interactions Expert



Deliverables

User-friendly deliverables with analyzed results make you save time.

The results of each ULTImate screen (ULTImate Y2H™, MBmate Y2H, ULTImate Y1H, ULTImate RNA Y3H or ULTImate YCHemH) are presented to you fully analyzed in three complementary files:

  • A Results summary listing the protein partners identified, the start and stop position of each fragment found and the confidence score of each interaction (the Predicted Biological Score, PBS®)

  • An Excel® spreadsheet containing the Genbank accession number for each partner, the relevant community ID such as those from Flybase, Wormbase, TAIR etc. and the raw sequences for each clone

  • A ‘DomSight’ pdf file providing for each partner a graphical comparison of the experimental interacting domain, the SID®, with known functional and structural domains. In a simple look, get more insights into the role and the significance of the interaction.

Graphical comparison of protein interacting domains

An example of each results file can be provided upon request, contact us!

Benefit from our exclusive Predicted Biological Score: PBS®

Because our libraries are screened to saturation as part as our unique ULTImate process, Hybrigenics is able to assign a statistical confidence score, the Predicted Biological Score (PBS®) to each interaction. Interacting proteins are ranked thanks to both local and global technical parameters. Locally, the number of independent fragments coding for the same protein, as well as their start and stop positions, are taken into account. Globally, information derived from all screens performed at Hybrigenics on all libraries from the same organism, are used to compute the final score. This includes reciprocal interactions, 3- or 4-cycles of interacting proteins, and the tagging of highly connected domains.

The PBS is computed as an e-value and thresholds are attributed to define categories from high confidence (A) to lower confidence (D) interactions. E and F are distinct categories flagging highly connected domains and technical false positives, respectively.

For a complete description of our confidence score, please have a look at the following publications: Rain et al., Nature 2001; Formstecher et al., Genome Research 2005; Formstecher et al. Supplemental Data.

Take advantage of our unparalleled experience with more than 9,000 screens conducted on over 35 species :

  • work on reliable interactions, not artefacts
  • save time on the analysis of your screen results
  • speed up your functional validation

Project Follow-up

In addition to this comprehensive data analysis, your dedicated project leader is available after the results delivery to review each interaction, answer your questions and share his / her technical expertise.

And as discussing science face to face is always easier, our teams will be pleased to visit you!

Download here information on how to read your result files.

Want to combine your screen results with protein interactions from the literature or your own interaction data? Want to save time in your analysis with direct access from the results to PubMed, GenBank and the community resources that matter to you – such as Flybase, Wormbase, TAIR, Zfin…?

Please have a look at our PIMRider page. PIMRider® is our dedicated software to visually explore protein interaction maps – and prepare publication-grade snapshots!

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