My laboratory carried out several two-hybrid screens, using a human placenta library. We designed the baits, and all of them but one gave great results, that were (and are being) validated as physiologically relevant by in vitro binding assay and co-IP assays. So, we trust very much all the results obtained in these Y2H screens. The service is fast and efficient, and the results are communicated in a clear manner. I enthusiastically recommend the services of Hybrigenics to any researcher wishing to discover novel interactors for their protein(s) of interest.
Sandra Citi (University of Geneva, Geneva, Switzerland) -