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ULTImate Y1H (yeast one-hybrid) uses a DNA sequence as a bait.
Derived from our ULTImate Y2H™ technology, it allows to screen to saturation highly complex libraries to find the protein partners of a DNA bait.
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To conduct an ULTImate Y1H assay, a DNA sequence of interest, the ‘DNA bait’, is first cloned upstream of a reporter gene to create a ‘DNA bait - reporter’ construct.
The DNA bait attached to the reporter is cloned into a centromeric vector or integrated into the genome of a dedicated yeast strain by site-specific recombination. A cell-to-cell mating is then performed between the ‘DNA bait - reporter’ yeast strain and a yeast strain pre-transformed with a high complexity domain library. This allows screening for novel protein partners of this DNA bait in an exhaustive fashion.
The results of your ULTImate Y1H screen come fully analyzed to help you focus on the most relevant interactions. The deliverables include the list of identified protein partners, the experimental interaction domain on each protein and a confidence score. Please click here for a detailed presentation.