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ULTImate RNA Y3H (RNA yeast three-hybrid) for RNA bait screening.
Derived from our ULTImate Y2H technology, it allows to screen highly complex domain-based libraries to identify protein partners of any short RNA.
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ULTImate RNA Y3H is based on ULTImate Y2H, with modifications to use a small RNA as a bait. It consists of the detection of ‘bait-prey’ interactions thanks to the reconstitution of a full transcription factor from DNA Binding Domain and Activation Domain moieties. In the RNA Y3H approach, three hybrid molecules are used:
Upon interaction of the RNA bait with the prey, the gap between the DBD and the AD is bridged, enabling the transcription of the reporter gene in yeast cells. The DNA of the positive clones is then sequenced and analyzed to identify the protein partners.
The results of your ULTImate RNA Y3H screen come fully analyzed to help you focus on the most relevant interactions. The deliverables include the list of identified protein partners, the experimental interaction domain on each protein and a confidence score. Please click here for a detailed presentation.